Now showing items 1-10 of 14
Metabolic engineering of Escherichia coli and Saccharomyces cerevisiae to mutasynthesize natural phenylpropanoids and novel analogs
The current dissertation investigated the biosynthesis of natural and unnatural flavonoids using Escherichia coli and Saccharomyces cerevisiae. Flavonoids are a diverse group of secondary metabolites found ubiquitously in ...
Recycling acetate for improving flavanones production from Escherichia coli
Acetate is a toxic by-product of glucose metabolism in Escherichia coli. In the present work, endogenous metabolic networks of E. coli were re-designed to improve the autonomous recycling of acetate into acetyl-CoA. In ...
Design and analysis: Scaling up of bioreactor towards directed differentiation of human embryonic stem cells
Embryonic stem cells will be an effective tool as a renewable source in regenerative medicine mainly due to their extensive proliferation and ability to differentiate into various lineages of cells. Widespread use of ...
Tissue engineering for cardiovascular regeneration - Stem cells, Biomaterials, and Animal preclinical model
This thesis addresses some approach for tissue engineering in cardiovascular regeneration. Cell source, biomaterials, ex vivo and in vivo assessment are all discussed step by step. This dissertation provides a good ...
Robust microbial fabrication of plant pharmaceuticals: Complexities and solutions
Abstract not available.
Matrigel and Mass Spectrometry
Matrigel is one of the most prolific 3-D growth cultures used for tumor invasion assays, tissue engineering and for stem cell growth. Produced from the Engelbreth-Holm-Swarm (EHS) tumor sarcoma cells of mice and containing ...
Impairment in the Ubiquitin Proteasome System in hereditary peripheral neuropathies
In several neurodegenerative diseases in which misfolded proteins accumulate there is impairment of the ubiquitin proteasome system (UPS). We tested if a similar disruption of proteostasis occurs in hereditary peripheral ...
Chemical and Biological Attenuation Methods for Bacterial Mediated Gene Delivery
The development of nonviral vectors for safe and effective gene delivery system is acquiring significant consideration at the present time. Ideally a nonviral vector must overcome the barrier of extracellular milieu, ...
Mathematical Modeling of N-Glycosylation: Model Construction and Parameter Estimation from Mass Spectra of Chinese Hamster Ovary Cell Mutants
Colossal advances in molecular cellular biology have witnessed the growth of genomics, proteomics and other "–omics" fields, built on reductionist approaches aimed at annotating a vast array of biological components, from ...
Mannosylated bio-synthetic hybrids for targeted antigen presenting cell gene delivery
The aim of gene delivery research is to develop clinically pertinent vectors that can be used to combat elusive diseases. Moreover, gene modulation efficacy of nonviral vectors have been hindered by numerous in vitro and ...