Now showing items 31-40 of 164
The energetics of neuromuscular acetylcholine receptor gating
Nicotinic neuromuscular acetylcholine receptors (nAChRs) are members of the pentameric ligand-gated ion channel family and function as signal transducers. The driving force for the opening conformational change is a ∼6000 ...
Tissue engineering for cardiovascular regeneration - Stem cells, Biomaterials, and Animal preclinical model
This thesis addresses some approach for tissue engineering in cardiovascular regeneration. Cell source, biomaterials, ex vivo and in vivo assessment are all discussed step by step. This dissertation provides a good ...
Genetic determinants of mitochondrial function and dysfunction
Maintenance of the mitochondrial genome is essential for respiration and other mitochondrial functions which are ultimately required for stability of the nuclear genome. In this work, I investigated several genetic ...
Interactions between papillomavirus E1 and E2 proteins and cellular replication factors modulate each others functions
Along with the viral proteins E1 and E2, papillomavirus DNA replication is carried out by the cellular DNA replication machinery. Many of these cellular replication factors have been identified using a viral cell-free DNA ...
Analysis of the biosynthesis and function of a serotype B glycoform of Moraxella catarrhalis lipooligosaccharides
Moraxella catarrhalis is an important human pathogen and a major cause of otitis media in children and respiratory infection in adults with chronic obstructive pulmonary disease (COPD). Few virulence factors have been ...
Centrosomally localized EVI5 is a novel GTPase activator of the small GTPase Rab11 and may play crucial roles in cytokinesis and neuroblastoma development
Neuroblastoma is a disease that develops due to the failure of neural crest cells to migrate and differentiate properly. The human EV15 gene was isolated and characterized as a result of its location at the breakpoint ...
Development and application of methodology for rapid NMR data collection and protein structure determination
This thesis focuses on the development and application of methodology for rapid NMR data collection and protein structure determination. In chapter 1, simultaneously cycled (SC) NMR is introduced and exemplified by ...
Yeast two-hybrid interaction partner screening through in vivo Cre-mediated binary interaction tag generation
Yeast two-hybrid (Y2H) is the tool of choice for the identification of novel protein-protein interactions, and it has been successfully used for genome-wide screens with several model organisms. Nonetheless, the logistics ...
Vitamin C and collagen IV formation in fibroblasts
Collagen IV (Col4) is a major component of vascular basement membranes (BM). Mice expressing mutated Col4 died shortly after birth with cerebral hemorrhage. Mice lacking the ubiquitous vitamin C (Asc) transporter SVCT2 ...
Robust microbial fabrication of plant pharmaceuticals: Complexities and solutions
Abstract not available.