Now showing items 1-10 of 16
Studies of structure, hydration and dynamics of biological macromolecules by use of NMR spectroscopy
Nuclear magnetic resonance (NMR) has emerged as a powerful tool for studying biological macromolecules in aqueous solution. This dissertation describes the efforts to study the structure, hydration and dynamics of biological ...
Role of the Escherichia coli beta-clamp, RecA activation and the SOS response in DNA polymerase selection
Error-prone DNA polymerases (pols) are conserved in all three kingdoms of life to promote the potentially mutagenic process of translesion DNA synthesis (TLS) following DNA damage. Due to their reduced fidelity, organisms ...
Environmental and binding dependence of protein terahertz dielectric response
Terahertz (THz) generation and detection techniques have been developed widely and improved in the last few decades. THz techniques have been applied to many fields, such as medical support (THz imaging for breast cancer ...
The response of proteasome activator 200kD to DNA damage
Upon exposure to DNA damaging agents, eukaryotic cells employ a complex cellular response, which includes cell signaling and protein recruitment events, in order to sense and repair DNA damage. The correction of DNA ...
Recycling acetate for improving flavanones production from Escherichia coli
Acetate is a toxic by-product of glucose metabolism in Escherichia coli. In the present work, endogenous metabolic networks of E. coli were re-designed to improve the autonomous recycling of acetate into acetyl-CoA. In ...
Structural characterization of JAA-F11-Fab by X-ray crystallography
Thomsen Friedenreich antigen (TF-Ag), is a carbohydrate antigen found on many types of tumors, including breast, prostate, bladder and ovarian. Passive transfer of JAA-F11, a monoclonal anti-TF-Ag antibody, has been shown ...
Vitamin C and collagen IV formation in fibroblasts
Collagen IV (Col4) is a major component of vascular basement membranes (BM). Mice expressing mutated Col4 died shortly after birth with cerebral hemorrhage. Mice lacking the ubiquitous vitamin C (Asc) transporter SVCT2 ...
Robust microbial fabrication of plant pharmaceuticals: Complexities and solutions
Abstract not available.
Molecular mechanisms regulating DeltaNp63 expression and function during development and differentiation of mouse skin keratinocytes
The skin is a multilayered stratified epithelium providing a protective barrier against dehydration and pathogenic insult. The transcription factor p63 plays an important role in the development of stratified epithelium ...
Study of the effect of tungsten shields on the radiation dose delivery in a gynecological applicator using Gafchromic film dosimetry
Fletcher Suit Delclos applicator is employed for high dose rate intracavitary brachytherapy treatment of cervical cancer. It includes tungsten shields in the direction of bladder and rectum to limit their radiation exposure. ...