Show simple item record

dc.contributor.authorSmonskey, Matthew Thomas
dc.date.accessioned2016-03-21T20:13:47Z
dc.date.available2016-03-21T20:13:47Z
dc.date.issued2007
dc.identifier.isbn9780549173175
dc.identifier.other304768505
dc.identifier.urihttp://hdl.handle.net/10477/42680
dc.description.abstractConjugated linoleic acid (CLA) prevents mammary tumors in rats. The purpose of this study was to test the hypothesis that common pathways are altered by two similarly chemoprotective isomers, 9,11-CLA and 10,12-CLA. Microarray analysis of rat mammary glands after CLA feeding demonstrated altered expression of genes involved cellular growth, death, and cancer development. Validation by real-time PCR was performed on mammary tissues for the following genes: Adam17, Agt, Bin1, Btg2, Casp2, Dgka, Ets1, Gas5, Hmgb2, Krt4, Psmb9, Stat1, Tp53, and Vdac1. Most genes validated by RT-PCR showed a pattern of change similar to that seen by microarray. Lastly, a study was conducted to determine if whole gland changes are represented in a fibroblast population. RT-PCR of CLA-treated rat mammary stromal vascular cells demonstrated a distinct pattern of gene expression from whole gland, suggesting that fibroblasts may not be the primary target of CLA induced changes observed in the microarray.
dc.languageEnglish
dc.subjectBiological sciences
dc.subjectConjugated linoleic acid
dc.subjectChemoprevention
dc.subjectBreast cancer
dc.subjectMicroarray
dc.subjectRT-PCR validation
dc.subjectMammary stromal-vascular cells
dc.titleMicroarray and real time-polymerase chain reaction analysis of mammary glands from rats fed conjugated linoleic acid
dc.typeDissertation/Thesis


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record