Effect of conjugated linoleic acid on motility and invasion of breast cancer epithelial cells
Siddiqui, Saima S.
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Conjugated linoleic acid (CLA) is a dietary fatty acid which inhibits the development, angiogenesis, and metastasis of mammary tumors. The purpose of this study was to test the effects of 9,11- and 10,12-CLA isomers on motility of MDA-MB-231 breast cancer cells. An in vitro wound healing assay demonstrated a significant decrease in motility in the presence of the 10,12-CLA isomer. Cell viability was altered but not at the same concentrations at which decreases in cell motility were seen. Transwell motility assay also showed an inhibitory effect of 10,12- but not 9,11-CLA. Videomicroscopy demonstrated a significant decrease in cell motility in the presence of both isomers. These results suggest that 10,12-CLA can decrease motility of MDA-MB-231 cells in response to a stimulus ( in vitro wound healing assay) as well as the baseline migration assay under conditions where no stimulus was applied (transwell motility assay and videomicroscopy).