Toward the development of resorbable laminated repair membranes
Tan, Loraine T.
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This dissertation centers on determining the physicochemical properties within biodegradable polymer (BP) platforms as a function of formulation chemistry and composition. The results of this research can be used to guide the development of custom tailored materials/formulations that allow one to stabilize sequestered proteins and provide active proteins upon liberation from a Resorbable Laminated Repair Membrane (RLRM) for use as drug-delivery devices. This dissertation describes our approach to characterize the physicochemical properties by using steady-state and time-resolved fluorescence spectroscopy. A description of the automated methods utilized for the construction and rapid screening of BP libraries is provided. To determine local microenvironment pH within a BP platform, the effect of tethering a pH sensitive probe, carboxy SNARF-1, to dextrans of varying MW and to a protein, in buffers of varying pH is explored. The effects are assessed by determining the pK a of the multiple fluorescent probes using steady-state fluorescence. This dissertation also describes the use of epi-fluorescence microscopy to investigate the spatial dependent pH change of the BP platform during hydrolysis.