Effect of lanthanides on Porphyromonas gingivalis proteases and dentinal hypersensitivity
Sunkara, Sasi Kumar
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Objective. Host and bacterial proteases play a vital role in periodontitis. Inhibitors of these proteases are necessary for control of this disease. The purpose of this study was to evaluate the effect of lanthanides on Porphyromonas gingivalis (P.gingivalis) proteins, a major pathogen in periodontitis. Methods. BAPNA (Benzoyl-L-Arg-p-nitroanilide); H-Gly-Pro-pNa HCl and gelatin were used to evaluate the activity of P.gingivalis proteins in presence of lanthanides. Proteins extracted from cell surface and culture media of P.gingivalis were assessed for their activity by different lanthanides for BAPNA assay. However, only gadolinium chloride was used for H-Gly-Pro-pNa HCl assay and gelatin-zymography. Results. Concentration dependent reduction of absorbance was observed in the presence of lanthanides with BAPNA and a similar observation was made with gadolinium chloride using H-Gly-Pro-pNa. Collagenolytic activity in cell surface extract and culture media precipitated proteins was absent in the presence of gadolinium chloride. Conclusion. These results suggest that the lanthanide, gadolinium, can be a potential inhibitor of P.gingivalis proteases.