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dc.contributor.authorBartos, Jeremy David
dc.date.accessioned2016-03-28T19:06:45Z
dc.date.available2016-03-28T19:06:45Z
dc.date.issued2005
dc.identifier.isbn0542125439
dc.identifier.isbn9780542125430
dc.identifier.other305363142
dc.identifier.urihttp://hdl.handle.net/10477/44863
dc.description.abstractGenomic instability is one of the hallmarks of tumorigenesis, although it is still disputed if this process acts as a progression facilitator, or is a secondary consequence of malignancy. Intrachromosomal genomic instability has been detected in the pre-malignant aberrant crypts of the colon, supporting the model that instability is an early step in the progression to malignancy in sporadic colorectal tumors and is therefore most likely a facilitator of tumorigenesis. Intrachromosomal genomic instability was quantified using Inter-(Simple Sequence Repeat) PCR analysis on paired tumor and normal DNA samples of breast, thyroid, and colorectal cancers, ultimately quantifying similar overall levels of instability. We assessed the correlations between K-ras and GSTM1 mutations and a tumor's level of genomic instability. A significant association was found between tumors with a GSTM1-null status and elevated inter-(simple sequence repeat) FCR instability, while a weak correlation was observed between mutant K-ras and inter-(simple sequence repeat) PCR genomic instability. Colorectal tumors were analyzed using comparative genomic hybridization BAC microarrays. There is a three BAC-clone region on chromosome 9 that contains the c-ABL gene, where copy number alteration is associated with genome-wide aCGH copy number loss. There is a highly significant interaction between that region of chromosome 9 and chromosome 22q11-13, another region whose loss is associated with genome-wide deletion. Additional regions of interest include the telomeric end of chromosome 14 whose loss is associated with genome-wide copy number losses, and regions on chromosome l, 2, 3, 7, and 13 whose amplification is associated with genome-wide copy number gains.
dc.languageEnglish
dc.sourceDissertations & Theses @ SUNY Buffalo,ProQuest Dissertations & Theses Global
dc.subjectHealth and environmental sciences
dc.subjectBiological sciences
dc.subjectColon cancer
dc.subjectIntrachromosomal
dc.subjectCancer genomes
dc.titleEvaluating sources of intrachromosomal instability in sporadic cancer genomes
dc.typeDissertation/Thesis


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