Pin-printing utility and feature characteristics on planar surfaces & mechanism of fluorescein corneal staining
Kraut, Nadine D.
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This thesis covers two areas of research both of which have spectroscopy at the center. The first part of the thesis investigates pin-printing strategies on planar surfaces. These surfaces include silicon based substrates such as glass microscope slides, mirrored glass slides, and oxidized porous silicon. Spectroscopy techniques are used to assess the results of pin-printed microarrays on these substrates. Topics investigated include stability, uniformity, and basic screening of the materials printed onto these surfaces. The second part of this thesis focuses on elucidating the mechanism behind solution induced corneal staining. Spectroscopy experiments are reported that investigate specific interactions that are occurring at the human corneal epithelial cell surface. Different interactions are probed by using molecular fluorescence spectroscopy.