Characterization of biodegradable amino acid-based Poly(ester amide) (AA PEA) hydrogels as biomaterials for tissue engineering
Best, Robert L.
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A new class of biodegradable polymers known as amino acid-based Poly(ester amide) (AA PEA) hydrogels have been synthesized and characterized for their performance as tissue engineering matrices for the delivery of growth factor to a wound site in order to aid in re-epithelialization of skin cells. Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) and X-Ray Photoelectron Spectroscopy (XPS) successfully determined characteristic information from the hydrogels to distinguish between polymer and protein signals in both current and future pharmacokinetic studies of growth factor uptake and release. These techniques also played a pivotal role in developing a supercritical CO 2 extraction to eliminate PDMS contamination from the hydrogels making them ready for use as tissue engineering matrices. Additionally, initial fluorescence results indicate that protein uptake may be optimized by incorporation of different amino acids into the polymer backbone. The results of this study will serve as a guideline for cleaning polymers to be used as biomaterial delivery systems as well as aid in the design of the optimal tissue engineering matrix of human growth factors.