In vitro impact of incorporating triclosan or cetylpyridinium chloride to bis-acryl provisional material
Garza Garza, Arely Denisse
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As dentists we are tasked to help patients maintain their natural dentition, restore lost or missing teeth, and promote oral health. During the fabrication of a definitive crown or fixed dental prosthesis provisional restorations are used. Reasons for using them are protection of pulpal tissue, prevention of dental caries, comfort, function and esthetics. Requirements for provisional restorations, among others, include good marginal adaptation, high polishability, strength, durability, and esthetics. The purpose of this project is to evaluate and quantify the release of antibacterial agents, triclosan or cetylpyridinium chloride (CPC), when added to bis-acryl provisional materials. The hypothesis is that the bis-acryl material with the addition of CPC or triclosan will have a predictable release of antibacterial activity when used as a provisional restoration for crown and bridge. Materials The bis-acryl resin used in this study includes two types: Systemp C&B (Ivoclar Vivadent, Amherst, NY) and Temphase (Kerr, Kerr, West Collins Orange, CA). The antibacterial agents include triclosan (Irgasan, Sigma-Aldrich Co. LLC.) and CPC (cetylpyridinium chloride, Sigma-Aldrich Co. LLC.). Three concentrations were selected as follows: triclosan 0.5%, 1% and 2 %; CPC .05% .01% and .15%. Aggregatibacter actinomycetemcomitans (Aa). Streptococcus gordonii (Sg) and Porphyromonas gingivalis (Pg), were the bacteria used to assess the effectiveness of the test material. Methods For this study, there were twelve groups with 90 samples each. Control samples consisted of disks measuring 10 mm in diameter. For the experimental group s, triclosan or CPC was added by weight. The amount of triclosan or CPC was determined to the nearest microgram to achieve the desired concentrations. The base, catalyst and antibacterial products were hand mixed with a spatula forming an homogenous mix and then loaded into silicon molds. All control samples and test samples were then tested against the three different bacteria to assess their antibacterial activity. Results : Visual inspection revealed that none of the control samples showed bacterial inhibition. There was no bacterial inhibition for samples containing triclosan with either SG or PG. No bacterial inhibition was found for samples containing CPC with AA. Samples containing triclosan showed a zone of inhibition with AA and samples containing CPC showed the same for SG and PG. The conclusion was that CPC targeted two bacteria vs. only one inhibited by triclosan. The results of this study also revealed that as concentration increased for both antibacterial agents, a larger zone of inhibition was present. Further research is needed to verify the physical properties of the materials developed.