Infrared microscopic monitoring of microfouling of germanium substrata
MetadataShow full item record
IR Microscopy can be used to non-invasively monitor micro-biofouling in 100 micrometer semiconductor channels in real time, with sensitivity to events ranging from 10 nanometer-thick 'conditioning' film deposition through 5-micrometer 'primary' bacterial films to full biofilms growing beyond 20-micrometer thicknesses. Diminished radiance is associated with suppression of convective heat transfer through hydrated 'conditioning' films at the solid liquid interface, while increased radiance accompanies film consolidation and dehydration allowing improved heat conduction from thermal source to semiconductor undersurface. Radiance patterns also observed in 'fab' water-exposed samples containing extremophilic microbes. Exemplary data will be shown for germanium infrared prisms exposed to surrogate microfouling by fibrinogen protein and hyaluronic acid 'conditioning' films, transferred bacterial lawns, and planar vs. depth calibration standards. Future work includes application to flow-cell systems and biosensor circuits.