Contractile behavior of field stimulated left ventricular myocytes from biopsy samples of the anterior and remote regions of the swine heart
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Dysfunctional (DYSF) myocardium refers to viable regions with reduced contractile function. DYSF myocardium was induced either in the anterior region or both in the anterior and remote regions of the left ventricle (LV) of swine heart by coronary artery occlusion. Single cardiac ventricular myocytes were obtained from the anterior and remote regions with DYSF myocardium. We first developed a successful isolation of calcium-tolerant myocytes using a biopsy method. Contractile properties including cell shortening, time-to-peak, and half-relaxation time of field-stimulated myocytes, and cell morphology including cell length and width were studied using a video edge detector. Our results suggest that increased percent of cell shortening in one region of the LV might be the result of a global change induced by an occlusion that produces DYSF myocardium in another region of the LV; and this increase might compromise cell viability or normal cellular function in the occluded region of the LV.