Bioanalytical applications of electrospray ionization mass spectrometry for proteome and biomarker analysis
Pennington, Christopher Lane
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Liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) has become a valuable tool for proteome and biomarker analysis. The work presented in this dissertation focused on the development MS based methods, applicable to the analysis of complex biological samples, so that proteomic and biomarker analysis of human urine and plasma samples could be performed. Much of this work is comparative in nature, between the chosen subject pool (autistic or celiac patients) and their relevant controls. Results from the ESI-LC-MS analysis of urine and plasma for gluten exorphin peptides and other target peptides are presented. Additionally, work toward the development of an enzymatically based tyrosine isotopic labeling strategy is discussed.