Mitochondria-wide screening of genes involved in protection against arsenic toxicity in Saccharomyces cerevisiae
Shroff, Meghna R
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Epidemiological studies have suggest a significant increase in the risk of various cancers on exposure to arsenic. The mechanism(s) of arsenic carcinogenesis in humans is poorly understood. In this research, we have employed yeast Saccharomyces cerevisiae as an important tool to study the mechanisms of carcinogenicity of arsenic in humans. We screened a total of 466 single gene deletion mutants of S. cerevisiae , known to be involved in various mitochondrial processes, to identify nuclear genes that may be affected in response to arsenic. We identified 72 arsenite sensitive mutants and 81 arsenate sensitive mutants. We categorized the identified genes based on mitochondrial processes including transcription, signal transduction, metabolism and vacuolar processes. We analyzed the data using PathwayAssist ® to obtain interactions among these genes. Further, we found human orthologs to the identified yeast genes. Together these studies reveal conserved pathways, which protect cells from arsenic-induced cell death in yeast and humans.