An investigation of bovine intracranial endothelial cell behavior through isolation and characterization including response to shear stress
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Endothelial cells play a vital role in the physiology and pathophysiology of the vasculature. The cerebrovasculature region is populated by endothelial cells with distinct structures and functions. To study how cerebrovascular endothelial cells differ in comparison to other vascular beds it is necessary to use cells from specific cerebrovascular regions. The first part of this thesis describes a method for culturing primary endothelial cells from bovine cerebral arteries. Endothelial identity was confirmed by the ability to uptake low-density lipoprotein and the expression of Factor VIII and eNOS. Cell-line suitability was confirmed by the ability to form confluent monolayers. The second part of this thesis directly compares the newly characterized primary endothelial cells with endothelial cells from extracranial sources. As reactive oxygen species (ROS) modulate endothelial functions through intracellular signaling, ROS generation was measured between cell populations under conditions of normal and elevated wall shear stress. Under chronic application of either regimen, intracranial endothelial cells generated significantly greater ROS compared with similarly treated aortic endothelial cells. This confirms the presence of fundamental differences in cerebrovascular endothelial cells may indicate important signaling mechanisms specific to the cerebral vasculature.