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dc.contributor.authorHsu, Yun Hsiang
dc.date.accessioned2016-04-05T19:11:12Z
dc.date.available2016-04-05T19:11:12Z
dc.date.issued2013
dc.identifier.isbn9781303474378
dc.identifier.other1459751789
dc.identifier.urihttp://hdl.handle.net/10477/50266
dc.description.abstractThis dissertation presents experimental studies on two-dimensional ultrastructures in the plasma membrane of living cells. These ultrastructures are one strategy that cells uses to organize molecule that has functional importance. Because of the diffraction limit, the ultrastructure cannot be resolved by conventional microscope. A single particle tracking technique, thermal noise imaging, was established to study the ultrastructure on the cell membrane because of the simultaneous high spatial and temporal resolution provided by thermal noise imaging. In order to better understand the complex structure on the cell membrane, several enhancements on the thermal noise imaging microscope have been established. The improved 3D detector calibration based on local diffusion is one important enhancement which completely overcomes the non-linear signal response of the position detector (quadrant photo diode). Thermal noise imaging tracks a tracer (216 nm particle) attached to a diffusing membrane protein. Because of the trapping, the protein is confined in an area of about 300 nm by 400 nm, which gives sufficient data to map the local properties of the cell membrane. With the help of the completely linearized signal response, greater detail can be revealed on the maps of membrane stiffness and viscosity. Using the established analyses (primarily logarithmic normalized occupancy and local membrane stiffness map), transient cholesterol stabilized nano-domains and linear structures were detected. According to the result of the analyses, it suggests that those transient cholesterol stabilized nano-domains tend to form around the fence like linear structures (possibly actin filaments), and glycosylphosphatidylinositol (GPI)-anchored green fluorescent proteins tend to stay inside the cholesterol stabilized nano-domains
dc.languageEnglish
dc.sourceDissertations & Theses @ SUNY Buffalo,ProQuest Dissertations & Theses Global
dc.subjectBiological sciences
dc.subjectMembrane stiffness
dc.subjectMembrane structure
dc.subjectPlasma membranes
dc.subjectSingle particle tracking
dc.subjectThermal noise imaging
dc.titleHigh Resolution Maps of Cell Membrane Stiffness, Protein Diffusion and Distribution Characterized by Thermal Noise Imaging
dc.typeDissertation/Thesis


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