A clinical study to assess effect of oral hygiene with a probiotic organism on dental plaque pH and on plaque bacteria including Streptococcus mutans
MetadataShow full item record
The aim of this study was to evaluate the effect of 2 weeks oral hygiene with toothpaste containing Lactobacillus paracasei on dental plaque pH and on plaque bacteria including Streptococcus mutans. Forty subjects, both males and females, aged 18-65 years old were enrolled in this study and all of them were issued the same treatment. Oral samples of plaque (before and after sucrose challenge) and saliva were collected at the washout visit (after 2 weeks of oral hygiene with fluoride toothpaste), at the baseline visit (after 2 weeks of oral hygiene with placebo toothpaste) and at the final visit (after 2 weeks of oral hygiene with probiotic toothpaste containing heat-killed Lactobacillus paracasei ). In order to compare plaque pH before and after sucrose challenge, samples were diluted with 500ul of water and pH was measured and recorded. Saliva samples were diluted, plated on a selective agar of Streptococcus mutans and recorded. For statistic analyses, T-test was used, determining differences in numbers of S.mutans in saliva and in plaque pH at each phase of the study. Results showed no significant difference in numbers of S.mutans between washout and baseline (p>0.05) and a significant difference between washout and final visit and between baseline and final, which resulted in lower salivary levels of S.mutans (p>0.05). Sucrose challenge resulted in significant reduction in plaque pH at each evaluation (p<0.05). However, the pH reduction was higher after use of fluoride and placebo and lower after use of probiotic toothpaste. The results indicate that oral hygiene with L.paracasei -containing toothpaste for 2 weeks decreases the numbers of S.mutans in saliva and decreases plaque pH reduction after sucrose challenge, leading to a less acidic oral environment, preventing decalcification and consequently dental caries.