Effects of glucose and flow on reactive oxygen species in brain artery endothelial cells
Mele, Stephen Louis
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Endothelial cells play a vital role in the normal physiology of the vasculature. The cerebrovascular region is highly populated by endothelial cells with distinct morphology and functions. However, endothelial cells are also a vital region in the pathophysiology of the vasculature, such as aneurysm formation, due to reactive oxygen species (ROS) production. To study the effects of glucose and flow on ROS production in brain arterial endothelial cells, ROS production was measured. This thesis is divided into three parts: glucose effect on ROS, flow effect on ROS, and glucose effect on flow-induced ROS. Previous endothelial cultures were provided by Joeseph Moran-Guiati and Jason Kushner. The effect of high glucose on static endothelial cells was shown to increase ROS production as compared to the effect of normal glucose. Under chronic treatment of endothelial cells with high flow, ROS production was significantly greater that in endothelial cells under chronic treatment of normal flow. High glucose was shown to exacerbate the high flow response. These studies provide insight to a possible connection between intracranial aneurysm formation and a major risk factor, Diabetes Mellitus.