Stercobiln quantification via high resolution mass spectrometry
Rudolph, Heather L.
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Stercobilin is a mammalian metabolite excreted in both urine and in feces. It is a robust and stable biomolecule in various storage conditions and for long lengths of time. Due to its stability, stercobilin makes an ideal candidate to measure human waste contamination in public swimming facilities. In addition to its use as a waste marker in public swimming facilities, stercobilin has also been previously examined as a potential biomarker for Autism Spectrum Disorder (ASD) diagnosis. Previous research has demonstrated a qualitative decrease in stercobilin presence in ASD urine samples compared to control urine samples. Using high resolution and high mass accuracy mass spectrometry, stercobilin will be examined in swimming pool, urine and ASD mice feces matrices. In addition to measuring stercobilin via mass spectrometry techniques, methods to isotopically label stercobilin for use as a surrogate standard will also be explored for future quantitative analysis and detection of stercobilin in swimming pools, urine, and feces.