Xanthohumol, a nature prenylated chalconoid, selectively inhibits the profiferation of neurotumor cells (NG108-15)
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Aim: This study aims to investigate the effect and mechanisms of Xanthohumol (Xn), a nature prenylated chalconoid from hops and beer, on the proliferation and apoptosis of neurotumor NG-108 cells. Main method: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and typan blue staining were used to measure cell viability. The intracellular levels of reactive oxygen species (ROS) was measured by the florescent probe 2',7'-dichlorofluorescein diacetate (H2DCF-DA). Flow cytometry was performed to measure the cell cycle distribution. Protein expression was detected by western blot analysis and immunofluorescence. Key findings: Among the different dose examined, Xn showed the best inhibitory effect on the proliferation of NG-108 cells with IC50 of 40uM. Xn treatment arrested the cell cycle at G1/S stages. Further studies demonstrated that Xn treatment triggered the phosphorylation of the estrogen regulated kinase (ERK1/2) and p38 mitogen activated protein kinase (MAPK). However, the phosphorylation of c-Jun N-terminal protein kinase (JNK) was not altered by Xn. Intriguingly, the inhibitor of ERK1/2 but not p38 MAPK abrogated the NG108 cell cycle retarded by Xn. There is no apoptosis of NG-108 by Xn treatment. Conclusion and Significance: Xn treatment inhibits the proliferation of NG-108 cells via activating ERK1/2 signaling pathways, Xn could be a potential therapeutic natural product for neurotumor.