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dc.contributor.authorNabi, Erik
dc.date.accessioned2016-04-05T19:59:16Z
dc.date.available2016-04-05T19:59:16Z
dc.date.issued2015
dc.identifier.isbn9781339104515
dc.identifier.other1723353966
dc.identifier.urihttp://hdl.handle.net/10477/51588
dc.description.abstractProstate specific antigen (PSA) is a well-known biomarker utilized in the diagnosis and management of prostate cancer. PSA is a 33kDa member of the human tissue kallikrein family, exhibiting enzymatic-activity with chymotrypsin-like specificity. PSA is produced by the prostate epithelia and secreted into seminal fluid where it functions in liquefaction of seminal coagulum. Reports have suggested PSA has anti-tumor and anti-angiogenic properties. Serum PSA levels have been found to inversely correlate with prostatic disease progression and prognosis, while tissue PSA levels have been found to exhibit a direct correlation. In prostate cancer cells, high expression of PSA correlated with decreased population growth rates, induction of apoptosis, and reduced tumorigenicity. PSA has also demonstrated inhibition of endothelial cell proliferation, tube formation, and migration. In our lab, PSA has been shown to retain anti-tumor properties when enzymatically inactivated, and modulate angiogenesis-related growth factors in PC-3M and LNCaP prostate cancer cell lines. Interferon-β (IFN-β) is an immunoregulatory cytokine produced by all human cell types in response to a challenge. IFN-β has exhibited a wide range of anti-viral, anti-angiogenic, anti- tumor, and immunoregulatory functions. The cellular effects of IFN-β are primarily mediated through the modulation of target gene transcription. IFN-β has been utilized in the treatment of a broad spectrum of diseases, such as multiple sclerosis, glioma, fibrosarcoma, breast and prostate cancers. The anti-tumor activity of IFN-β has been demonstrated in the inhibition of angiogenesis, tumor growth, and metastasis of prostate cancer tumors in nude mice. IFN-β has also been shown to inhibit endothelial cell tube formation in fibrin gel assay. Hyperthermia is exposure to temperatures in excess of normal body temperature (37°C). Reports of clinical hyperthermia temperatures utilized in the literature range as high as 45°C. Hyperthermia has been found to have anti-tumor effects, particularly as a chemosensitizing adjuvant therapy to chemo- and radiation therapy. Our lab is particularly interested in fever- range hyperthermia (37°C), and the efficacy of its use in cancer therapy. The purpose of this study was to 1) Discern if PSA-derived peptides retained the anti- angiogenic properties of f-PSA in endothelial cell tube formation, migration, and target gene transcription modulation, and 2) Analyze target angiogenesis-related gene modulation in the PC- 3M epithelial prostate cancer cell line and in the HUVEC endothelial cell line
dc.languageEnglish
dc.sourceDissertations & Theses @ SUNY Buffalo,ProQuest Dissertations & Theses Global
dc.subjectBiological sciences
dc.subjectHealth and environmental sciences
dc.subjectAngiogenic assay
dc.subjectGene expression
dc.subjectInterferon
dc.subjectProstate cancer
dc.subjectProstate-specific antigen
dc.titleRole of PSA and interferon in prostate cancer gene expression and neovascularization
dc.typeDissertation/Thesis


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