MicroRNA based nanoparticles in lung cancer therapy and diagnosis
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This thesis is focus on the microRNA based nanoparticles in lung cancer therapy and diagnosis. We demonstrate a multifunctional liposome based delivery system for therapy and diagnosis. MicroRNA-29b is successfully delivered into A549 lung cancer cells and induces inhibition of cell growth. Gadolinium as a contrast reagent in magnetic resonance imaging is conjugated onto the liposome and the capability is examined. Pilot study of exosomes delivery system is conducted by delivering microRNA-486 into A549 cells. The results illustrate the therapeutic potential of miRNA encapsulated by exosomes. We also develop a novel microfluidic platform for early cancer detection by characterizing the surface protein of exosomes.