Contact Pin-Printing onto Porous Silicon for Creating Microarrays with High Chemical Diversity and Investigating the Effects of Commercial Antimicrobial Agents on Human Corneal Epithelial Cell Membranes
Horner, Ian J.
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This thesis covers two areas of research with spectroscopy being the commonality. The first part of this thesis investigates a porous silicon (pSi) microarray sensor for selective analyte detection. Towards this goal research focused on fabricating a pSi microarray, chemically modifying a pSi microarray, and determining the nature of the analyte-dependent photoluminescence (PL) response from features (spots) on the pSi microarray. This thesis discusses the fabricated pSi microarray spot heterogeneity, strategies for controlling the spot heterogeneity, methods for chemically modifying the spot via an UV-initated hydrosilylation (HS) reaction, and factors that cause the observed analyte-dependent PL response. The second part of this thesis focused on determining the interactions between common commercial antimicrobial agents and the human corneal epithelial cell membrane. Spectroscopy experiments are reported that investigate these different interactions by using molecular fluorescence spectroscopy, dynamic light-scattering (DLS), and liquid-chromatography mass-spectrometry (LC-MS).