Hydrogels as Wound Healing Delivery Systems
Travis, Gail Lynn
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This project focuses on determining and quantifying protein uptake and release kinetics as part of a larger tissue engineering and wound healing study. Model proteins, including bovine serum albumin (BSA), myoglobin from equine skeletal muscle, retinol binding protein (RBP-4), and green fluorescent protein (GFP), were loaded onto and released from poly(2-hydroxyethyl methacrylate) (pHEMA) and modified pHEMA membranes to study the protein uptake and release kinetics. The model proteins were used as a surrogate for keratinocyte growth factor (KGF), a protein used to accelerate wound restitution. Ultimately, GFP was selected as the best KGF surrogate because it exhibits strong visible fluorescence, it is less expensive in comparison to KGF, and it is similar in size to KGF (27 vs 19 kDa. To follow the uptake and release we used fluorescence spectroscopy.