The Influence of Exercise and Vitamin D3 on Tumorigenesis and Diaphragm Muscle Function in an NNK Murine Model
Williams, Brian Thomas
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Vitamin D status and exercise are both linked to lung cancer as treatments to reduce tumorigenesis and improve cancer driven muscle cachexia. This thesis utilizes healthy and lung cancer models to determine the benefit of a Vitamin D3 (VD3) and exercise (EXC) on lung tumorigenesis, the control of breathing, and muscle function in situ and in vivo. Our lab has shown that high dose VD3 supplementation can improve diaphragm (DIA) muscle function, these studies add that dietary intervention to an exercise intervention, which has been shown to independently reduce tumorigenesis in cancer models. Utilizing a four week long healthy animal study and twenty week NNK lung cancer model study, we assessed the effect of 25 and 10,000 VD3 as well as combining exercise (EXC) or a control group with no exercise (CTR) to reduce lung tumorigenesis, and improve DIA and soleus (SOL) muscle function and the control of breathing. We found that 10,000 VD3 reduced DIA function, hypercapnic ventilatory response and increased DIA muscle atrophy signaling. EXC was protective when combined with 10,000 VD3 supplementation in SOL muscle function but not DIA muscle function or hypercapnic ventilatory response.