Design of Metalloporphyrin Liposome for Biomedical Applications
MetadataShow full item record
Compared with the porphyrin, metalloporphyrin compounds are more common in nature due to the quenching phototoxicity of porphyrin by the metal chelation. Meanwhile, the metal chelation confers diverse biomedical applications to the porphyrin molecules. Through conjugating metalloporphyrin with lyso-lipid, the obtained metallporphyrin-lipid can form the liposome bilayer structure, which is well-suited for theranostic applications. Motivated by the unique chemical property of metalloporphyrin, this thesis focuses on exploring the novel biomedical applications of metalloporphyrin liposome. Chapter 1 provides a comprehensive background on metalloporphyrin, especially reviewing existing metalloporphyrin nanoparticles for theranostic applications. Chapter 2 describes a novel and convenient method to stably attach His-tagged polypeptides to liposome based on the chelation between histidine and cobalt porphyrin lipid (CoPoP). The CoPoP immunogenic liposome is applied in the design of MPER vaccine against HIV disease. Chapter 3 demonstrates the capacity of CoPoP liposome as a multivalent antigens carrier and a potential universal vaccine against influenza is designed and evaluated. Chapter 4 introduces the synthesis of N-HPPH-lipid which can form a hydrated bilayer structure through the hydrogen bond between amine terminal and water molecules. When N-HPPH-liposome is chelated with Mn, an enhanced MR imaging can be observed. Chapter 5 presented a brief conclusion and some future directions. The metalloporphyrin nanoparticles represent a promising platform for developing theranostic functions. It is believed that number of versatile and useful metalloporphyrin nanoparticles will be designed and applied in the biomedical applications.