Development Of Strontium-Alginate Scaffolds To Improve Gingival Fibroblast Function
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Background: Peri-implantitis is a progressive destructive condition as a result of active inflammatory process, which affects soft and hard tissue resulting in bone resorption and loss of the supporting structures surrounding the dental implant. There is no ideal or standard treatment approach to manage peri-implantitis and this is still an active field of research. Strontium material has a dual mode of action by promoting osteoblast cell differentiation, as well as inhibiting osteoclast differentiation, which make it of a promising clinical use for treatment of peri-implantitis. Aim: To produce strontium alginate rings scaffolds serving as a local reservoir for local delivery of strontium to the affected dental implant and to test the cytotoxicity and the release of strontium material in cell culture model of human gingival fibroblast cells. Materials and Methods: Specially designed molds were fabricated and used for the development of the strontium alginate scaffolds with different strontium concentrations to be administered both directly and indirectly to the human gingival fibroblast cell culture. These scaffolds were also incubated and the release of strontium from the different scaffolds concentrations was analyzed. Results: All the tested strontium concentrations appear to be biocompatible with no cytotoxic or negative effects on the human gingival fibroblast cells with an increasing cell viability noted with increasing strontium concentration in a linear relationship. Strontium release from alginate scaffolds was measured and released material is also able to maintain fibroblast activity. Conclusion: Strontium appears to be non-cytotoxic to human gingival fibroblasts when administered within a novel ring-shaped alginate scaffold.