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dc.contributor.advisorLin, Qing
dc.contributor.authorWu, Hsuan-Yi
dc.contributor.author0000-0003-1244-4891
dc.date.accessioned2019-10-28T20:18:25Z
dc.date.available2019-10-28T20:18:25Z
dc.date.issued2019
dc.date.submitted2019-08-09 16:02:14
dc.identifier.urihttp://hdl.handle.net/10477/80629
dc.descriptionPh.D.
dc.descriptionThe full text PDF of this dissertation is embargoed at author's request until 2021-09-19.
dc.description.abstractBioorthogonal chemistry focuses on the study of chemical reactions that take place in living systems without interfering with native biological systems. These studies often answer different questions of interest among chemical biologists and organic chemist. The central theme of this dissertation is the development and application of bioorthogonal chemistry for site-specific modification of proteins for biocatalysis and protein stabilization. The amber codon suppression technique was used to incorporate the alkene-containing amino acid into proteins. These alkene-encoded proteins will participate in photoclick chemistry or photo-crosslinking reaction for conjugation with their reaction partners. Chapter 1 reviews current bioorthogonal chemistry, with emphasis on the development and optimization of bioorthogonal reactions for protein labeling. Chapter 2 focuses on the development of bottle brush copolymer-enzyme conjugates for potential use in biocatalytic cyclopropanation. Because enzymes generally have a low tolerance for organic cosolvents, we hypothesize that the polymer-enzyme conjugates will show improved protein stability in aqueous media containing organic co-solvents. Chapter 3 focuses on the protein cyclizations via bioorthogonal reactions. Chapter 4 describes a new method of forming an intramolecular covalent bond within protein structure to stabilize proteins in the reducing environment.
dc.formatapplication/pdf
dc.language.isoen
dc.publisherState University of New York at Buffalo
dc.rightsUsers of works found in University at Buffalo Institutional Repository (UBIR) are responsible for identifying and contacting the copyright owner for permission to reuse. University at Buffalo Libraries do not manage rights for copyright-protected works and cannot assist with permissions.
dc.subjectChemistry
dc.subjectPolymer chemistry
dc.subjectBiochemistry
dc.titleBioorthogonal Chemistry: Synthesis of Protein-Polymer Conjugates and Orthogonally Crosslinked Protein
dc.typeDissertation
dc.rights.holderCopyright retained by author.


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